Detección de la actividad de desoxiribonucleasa (DNasa) en cepas de Clostridium chauvoei / [Deoxyribonuclease activity detection in Clostridium chauvoei strains]
Rev. argent. microbiol
;
37(2): 87-8, Apr.-June 2005.
Artículo
en Español
|
LILACS-Express
| LILACS, BINACIS
| ID: biblio-1171757
ABSTRACT
Beta toxin of C. chauvoei has desoxiribonuclease (DNase) activity which is regarded as one of its virulence factors. The production of DNase was detected in strains isolated from bovines, using as controls C. chauvoei ATCC 10092, and C. perfringens Type A and C. septicum, both laboratory isolates. The enzyme activity was made evident on a DNA substrate observing the macroscopic degradation. A simple methodology was developed using a commercial medium for DNase test, with the incorporation of sterile horse serum. Each strain was streaked on the surface of the medium, incubated in anaerobic atmosphere at 37 degrees C for 48 hours. The plates were revealed with HCI 1 N. The appearance of a clear and transparent zone around and under the microbial growing was considered a positive reaction. Enzyme activity was detected in 10 of 12 strains and also in the controls. The serum addition to the commercial basal medium allows the optimum development of the microorganism showing the enzymatic digestion zone.
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Índice:
LILACS (Américas)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Español
Revista:
Rev. argent. microbiol
Asunto de la revista:
Microbiologia
Año:
2005
Tipo del documento:
Artículo
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