Evaluation of the Genotoxicity of Endodontic Materials for Deciduous Teeth Using the Comet Assay
Pesqui. bras. odontopediatria clín. integr
;
21: e0060, 2021. tab
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-1250451
ABSTRACT
ABSTRACT Objective: To evaluate genotoxicity of zinc oxide, P. A. calcium hydroxide, mineral trioxide aggregate and an iodoform paste using comet assay on human lymphocytes. Material and Methods: Two positive controls were used: methyl-methanesulfonate for the P.A. calcium hydroxide and mineral trioxide aggregate; and doxorubicin for the iodoform paste and zinc oxide. There were also two negative controls: distilled water for the P.A. calcium hydroxide and mineral trioxide aggregate; and DMSO for the iodoform paste and zinc oxide. Comets were identified using fluorescence microscopy and 100 of them were counted on each of the three slides analyzed per drug test. A damage index was established, taking into consideration the score pattern that had previously been determined from the size and intensity of the comet tail. Analysis of variance, followed by Tukey's test, was used to compare the means of the DNA damage indices. Results: The DNA damage index observed for mineral trioxide aggregate (7.08 to 8.58) and P.A. calcium hydroxide (6.50 to 8.33), which were similar to negative control index. On the other hand, damage index for zinc oxide (104.7 to 218.50) and iodoform paste (115.7 to 210.7) were similar to positive control index. Conclusion: Iodoform paste and zinc oxide showed genotoxicity at all concentrations used.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Diente Primario
/
Óxido de Zinc
/
Ensayo Cometa
/
Genotoxicidad
/
Pruebas de Mutagenicidad
Tipo de estudio:
Estudios de evaluación
/
Estudio pronóstico
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Pesqui. bras. odontopediatria clín. integr
Asunto de la revista:
Odontología
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Federal University of Ceará/BR
/
State University of Feira de Santana/BR
/
State University of Southwest of Bahia/BR
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