Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production
Electron. j. biotechnol
;
48: 29-35, nov. 2020. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1254696
ABSTRACT
BACKGROUND:
Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production.RESULTS:
In our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively.CONCLUSIONS:
Min pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Porcinos Enanos
/
Bacterias
/
Celulasa
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Canadá
/
China
Institución/País de afiliación:
Northeast Agricultural University/CN
/
University of Calgary/CA
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