Non-isotopic polymerase chain reaction methods for the detection of HIV-1 in Ugandan mothers and infants
AIDS (Lond.)
;
5(12): 1463-1467, 1991.
Artículo
en Inglés
| AIM
| ID: biblio-1256009
ABSTRACT
Two non-isotopic polymerase chain reaction (PCR) methods were evaluated by testing blood from 41 HIV-1-seropositive and 16 HIV-1 seronegative Ugandan mothers and 56 of their children (aged 0.5- 15.0 months). Amplification of HIV-1 sequences was performed in duplicate using a biotinylated primer pair to the gag region (SK 462-431) and nested primer pairs (JA 17-20) to the pol region of HIV-1. gag sequences were hybridized using a microtiter plate coated with the SK 102 probe followed by colorimetric detection using an avidin-horseradish peroxidase conjugate and tetramethylbenzidine/peroxide substrate. Pol sequences were detected on angarose gel stained with ethidium bromide. Results of HIV-1 PCR analysis showed that 40 out of 41 (98) seropositive mothers and 10 out of 29 (34) seropositive or Western blot-indeterminate children had detectable HIV-1 sequences. Our results suggest that non-isotopic PCR methods are sensitive; specific; and potentially useful in the early diagnosis of HIV-1 infection in developed and developing countries
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Índice:
AIM (África)
Asunto principal:
Reacción en Cadena de la Polimerasa
/
VIH
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
AIDS (Lond.)
Año:
1991
Tipo del documento:
Artículo
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