Amplification of urea detection based on pH-sensitive liposomes
Electron. j. biotechnol
;
52: 30-34, July. 2021. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1283487
ABSTRACT
BACKGROUND: This study aimed to develop an amplification method of urea detection based on pHsensitive liposomes. RESULTS: The urease covalently immobilized on the magnetic particles and the pH-sensitive liposomes encapsulating ferricyanide were added to the cyclic-voltammeter cell solution where urea was distributed. The conversion of urea into carbonic acid seemed to induce a pH decrease that caused a reduction in the electrostatic repulsion between the headgroups of weakly acidic 1,2-dipalmitoyl-sn-glycero3-succinate. The reduction induced the liposomes to release potassium ferricyanide that was encapsulated inside. The effects of urea concentration and pH value were investigated. A specific concentration (0.5 mg/mL) of the urea solution was set to observe the response. The activity of urease was reversible with respect to the pH change between 7 and 5. The sensitivity of this detection was almost identical to the comparable techniques such as an enzyme-linked immunosorbent assay and a field-effect transistor. CONCLUSIONS: In summary, the methodology developed in this study was feasible as a portable, rapid, and sensitive method.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Urea
/
Liposomas
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Corea del Sur
Institución/País de afiliación:
Seoul National University of Science and Technology/KR
Similares
MEDLINE
...
LILACS
LIS