Changes of renal histopathology and the role of Nrf2/HO-1 in asphyxial cardiac arrest model in rats
Acta cir. bras
;
36(6): e360607, 2021. tab, graf
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1284911
ABSTRACT
ABSTRACT Purpose To investigate the role of Nrf2/HO-1 in renal histopathological ailments time-dependently in asphyxial cardiac arrest (CA) rat model. Methods Eighty-eight Sprague Dawley male rats were divided into five groups of eight rats each. Asphyxial CA was induced in all the experimental rats except for the sham group. The rats were sacrificed at 6 hours, 12 hours, one day and two days post-CA. Serum blood urea nitrogen (BUN), creatinine (Crtn) and malondialdehyde from the renal tissues were evaluated. Hematoxylin and eosin and periodic acid-Schiff staining were done to evaluate the renal histopathological changes in the renal cortex. Furthermore, Nrf2/HO-1 immunohistochemistry (ihc) and western blot analysis were performed after CA. Results The survival rate of rats decreased in a time-dependent manner 66.6% at 6 hours, 50% at 12 hours, 38.1% in one day, and 25.8% in two days. BUN and serum Crtn markedly increased in CA-operated groups. Histopathological ailments of the renal cortical tissues increased significantly from 6 hours until two days post-CA. Furthermore, Nrf2/HO-1 expression level significantly increased at 6 hours, 12 hours, and one day. Conclusions The survival rate decreased time-dependently, and Nrf/HO-1 expression increased from 6 hours with the peak times at 12 hours, and one day post-CA.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Lesión Renal Aguda
/
Paro Cardíaco
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Año:
2021
Tipo del documento:
Artículo
Institución/País de afiliación:
Jeonbuk National University Hospital/KR
/
Jeonbuk National University/KR
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