Pathological findings and morphologic correlation of the lungs of autopsied patients with SARS-CoV-2 infection in the Brazilian Amazon using transmission electron microscopy
Rev. Soc. Bras. Med. Trop
;
54: e08502021, 2021. graf
Artículo
en Inglés
| LILACS
| ID: biblio-1288094
ABSTRACT
Abstract INTRODUCTION:
Electron microscopy (EM) is a rapid and effective tool that can be used to create images of a whole spectrum of virus-host interactions and, as such, has long been used in the discovery and description of viral mechanisms.METHODS:
Electron microscopy was used to evaluate the pulmonary pathologies of postmortem lung sections from three patients who died from infection with SARS-associated coronavirus 2 (SARS-CoV-2), a new member of the Coronaviridae family.RESULTS:
Diffuse alveolar damage (DAD) was predominant in all three patients. The early exudative stage was characterized principally by edema and extravasation of red blood cells into the alveolar space with injury to the alveolar epithelial cells; this was followed by detachment, apoptosis, and necrosis of type I and II pneumocytes. The capillaries exhibited congestion, exposure of the basement membrane from denuded endothelial cells, platelet adhesion, fibrin thrombi, and rupture of the capillary walls. The proliferative stage was characterized by pronounced proliferation of type II alveolar pneumocytes and multinucleated giant cells. The cytopathic effect of SARS-CoV-2 was observed both in degenerated type II pneumocytes and freely circulating in the alveoli, with components from virions, macrophages, lymphocytes, and cellular debris.CONCLUSIONS:
Viral particles consistent with the characteristics of SARS-CoV-2 were observed mainly in degenerated pneumocytes, in the endothelium, or freely circulating in the alveoli. In the final stage of illness, the alveolar spaces were replaced by fibrosis.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Brasil
/
SARS-CoV-2
Tipo de estudio:
Estudio diagnóstico
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fundação Centro de Controle de Oncologia do Estado do Amazonas/BR
/
Fundação Oswaldo Cruz/BR
/
Fundação de Medicina Tropical Dr. Heitor Vieira Dourado/BR
/
Hospital Universitário Getúlio Vargas/BR
/
Instituto Nacional de Pesquisas da Amazônia/BR
/
Universidade do Estado do Amazonas/BR
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