Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique
Rev. bras. cir. cardiovasc
;
36(3): 318-322, May-June 2021. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1288241
ABSTRACT
Abstract Introduction:
The mouse heterotopic cardiac transplant model has been extensively used to explore transplant immunity. Although the cuff technique facilitates the operation, the procedure remains difficult, and vessel eversion is the most difficult step. Cuff movement and everted vessel wall slippage are the main adverse factors in vessel eversion. Traditional strategies to prevent these factors focus on cuff fixation, while more steps or surgical instruments would be required.Methods:
According to the reported protocols and our experience, the vessel eversion skills were modified and used for transplantation. Cardiac grafts from C57BL/6(H-2b) or BALB/c(H-2d) mice were transplanted into C57BL/6(H-2b) mice. The operating times of recent 90 operations, which were divided into 9 groups according to their sequence, were summarized and analyzed.Results:
The mouse cervical cardiac transplantation was successfully performed by using the modified vessel eversion skills. The cuff movement, which is the most important adverse factor to prevent vessel eversion, was effectively prevented. In the recent 90 operations, the total operating time was 47.3±7.9 min and the success rate was 98%.Conclusions:
The modified surgical skills simplify the vessel eversion in mouse cervical cardiac transplantation with cuff technique, characterized by less cuff movement, fewer steps, and surgical instruments. Using these surgical skills, the transplant can be performed in a short time.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Trasplante de Corazón
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Rev. bras. cir. cardiovasc
Asunto de la revista:
Cardiología
/
Cirugía General
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Chinese Academy of Sciences/CN
/
Southern Medical University/CN
/
The Second Xiangya Hospital of Central South University/CN
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