Prevalence of carbapenemase production in Pseudomonas aeruginosa isolates causing clinical infections in Lagos University Teaching Hospital, Nigeria
Afr. J. Clin. Exp. Microbiol
;
22(4): 498-503, 2021.
Artículo
en Inglés
| AIM
| ID: biblio-1342265
ABSTRACT
Background:
Pseudomonas aeruginosa has been highly associated with carbapenem resistance in which carbapenemases has been suggested to be a major contributory factor. Hence the objective of this study was to phenotypically detect KPC-type carbapenemase, metallo-ß-lactamase and OXA-48 carbapenemase production in clinical isolates of P. aeruginosa in Lagos University Teaching Hospital (LUTH), NigeriaMethodology:
One hundred and seventy-one P. aeruginosa isolates consecutively recovered from clinical specimens of patients with infections at the Medical Microbiology and Parasitology laboratory of the hospital were identified using MicrobactTM 24E kit. Preliminary screening for carbapenem resistance was determined by the disc diffusion method on Mueller-Hinton agar using single discs of meropenem and imipenem. Phenotypic detection of carbapenemase production among carbapenem-resistant isolates was performed by the combination disc test of meropenem-phenylboronic acid (MRPBO) and meropenem-dipicolinic acid (MRPDP) as recommended by EUCAST 2013 guideline.Results:
Out of the 171 P. aeruginosa isolates, 35 (20.5%) were carbapenem non-susceptible (resistant) while carbapenemase production was detected in 27 (77.1%) of these carbapenem resistant isolates, and no enzyme was detected in 8 (22.9%). Of the 27 carbapenemase producing isolates, 22 (81.5%) produced MBL, 1 (3.7%) produced KPC, while 4 (14.8%) produced both KPC and MBL enzymes.Conclusion:
This study revealed that carbapenem resistance among P. aeruginosa clinical isolates in our institution is gradually increasing. The mechanism for this rise is associated with carbapenemases, with MBL being the major carbapenemase involved. There is the need to ensure strict compliance with the LUTH infection control guidelines in order to check the rising incidence of infection caused by carbapenem resistant P. aeruginosa
Texto completo:
Disponible
Índice:
AIM (África)
Asunto principal:
Pseudomonas aeruginosa
/
Hospitales de Enseñanza
/
Infecciones
Tipo de estudio:
Guía de Práctica Clínica
/
Estudio de prevalencia
/
Estudio pronóstico
/
Factores de riesgo
País/Región como asunto:
Africa
Idioma:
Inglés
Revista:
Afr. J. Clin. Exp. Microbiol
Año:
2021
Tipo del documento:
Artículo
Institución/País de afiliación:
Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos/NG
/
Department of Medical Microbiology and Parasitology, Lagos University Teaching Hospital/NG
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