Systematic engineering of the rate-limiting step of b-alanine biosynthesis in Escherichia coli
Electron. j. biotechnol
; 51: 88-94, May. 2021. ilus, tab, graf
Article
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| LILACS
| ID: biblio-1343452
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ABSTRACT
BACKGROUND:
Large amounts of b-alanine are required in fine chemical and pharmaceutical synthesis and other fields. Profitable and green methods are required for the industrial production of b-alanine.RESULTS:
Replacing endogenous panD of Escherichia coli with heterologous CgpanD from Corynebacterium glutamicum enabled b-alanine synthesis of 0.67 g/L by strain B0016-082BB. Overexpressing CgpanD on both plasmids and chromosomes to enhance the rate-limiting step improved the b-alanine titer to 4.25 g/L in strain B0016-083BB/pPL451-panD with a slighter metabolic burden. Growth factors were introduced by addition of yeast extract, and 6.65 g/L of b-alanine was synthesized by strain B0016- 083BB/pPL451-panD in the M9-3Y medium.CONCLUSIONS:
Enhancement of the rate-limiting steps in the b-alanine biosynthetic pathway, recruitment of the temperature-sensitive inducible pL promoter, and optimization of the fermentation process could efficiently increase b-alanine production in E. coli.Palabras clave
Texto completo:
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Índice:
LILACS
Asunto principal:
Beta-Alanina
Idioma:
En
Revista:
Electron. j. biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2021
Tipo del documento:
Article