Synthesis and Biological Properties of Silver Chloride Nanoparticles Using Cell-free Extracts of Aeromonas hydrophila and Antibacterial Activity against Drug-Resistant Bacteria
Braz. arch. biol. technol
;
64: e21210010, 2021. tab, graf
Artículo
en Inglés
|
LILACS-Express
| LILACS
| ID: biblio-1350256
ABSTRACT
Abstract Microorganisms have been studied as potential biological factories for the synthesis of silver nanoparticles. In the present study, the cell-free extract of Aeromonas hydrophila was used to synthesize silver nanoparticles because the extract has a dual role in reducing and stabilizing silver nanoparticles. In this study, silver nanoparticles were synthesized using Aeromonas hydrophila. Synthetic nanoparticles were examined using ultraviolet-visible spectroscopy, X-ray diffraction, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM) and X-ray diffraction (EDX) spectroscopy. In this study, antimicrobial properties of Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa and anti-cancer properties (MCF-7, HepG-2) of silver nanoparticles were investigated. The synthesis of silver nanoparticles was confirmed by ultraviolet-visible spectroscopy and X-ray diffraction. TEM images detected the spherical shape of nanoparticles of various sizes in the range of 1-20 nm. FT-IR analysis demonstrated that enzyme, protein and carbohydrate compounds can be proven as stabilizing agents on the surface of silver nanoparticles. The resulting nanoparticles had strong antibacterial activity against drug-resistant bacteria. Silver chloride nanoparticles were also toxic to MCF-7 and HepG-2 cancer cells. The green synthesis method is cost-effective, environmentally friendly and an easy alternative to conventional silver nanoparticle synthesis methods.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Idioma:
Inglés
Revista:
Braz. arch. biol. technol
Asunto de la revista:
Biologia
Año:
2021
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Irán
Institución/País de afiliación:
Ferdowsi University ‎of Mashhad/IR
/
Hakim Sabzevari University/IR
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