Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels
Hematol., Transfus. Cell Ther. (Impr.)
;
43(4): 499-506, Oct.-Dec. 2021. tab
Artículo
en Inglés
| LILACS
| ID: biblio-1350821
ABSTRACT
ABSTRACT Introduction:
Flow cytometry has become an increasingly important tool in the clinical laboratory for the diagnosis and monitoring of many hematopoietic neoplasms. This method is ideal for immunophenotypic identification of cellular subpopulations in complex samples, such as bone marrow and peripheral blood. In general, 4-color panels appear to be adequate, depending on the assay. In acute leukemias (ALs), it is necessary identify and characterize the population of abnormal cells in order to recognize the compromised lineage and classify leukemia according to the WHO criteria. Although the use of eightto ten-color immunophenotyping panels is wellestablished, many laboratories do not have access to this technology. Objective andMethod:
In 2015, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) proposed antibody panels designed to allow the precise diagnosis and characterization of AL within available resources. As many Brazilian flow cytometry laboratories use four-color immunophenotyping, the GBCFLUX has updated that document, according to current leukemia knowledge and after a forum of discussion and validation of antibody panels.Results:
Recommendations for morphological analysis of bone marrow smears and performing screening panel for lineage (s) identification of AL were maintained from the previous publication. The lineage-oriented proposed panels for B and T cell acute lymphoblastic leukemia (ALL) and for acute myeloid leukemia (AML) were constructed for an appropriate leukemia classification.Conclusion:
Three levels of recommendations (i.e., mandatory, recommended, and optional) were established to enable an accurate diagnosis with some flexibility, considering local laboratory resources and patient-specific needs.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Leucemia
/
Citometría de Flujo
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
/
Estudio pronóstico
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Hematol., Transfus. Cell Ther. (Impr.)
Asunto de la revista:
Hematologia
/
TransfusÆo de Sangue
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Hospital Erasto Gaertner/BR
/
Hospital do Câncer de Barretos/BR
/
Sabin Medicina Diagnóstica/BR
/
Universidade Estadual de Campinas - UNICAMP/BR
/
Universidade Federal de Santa Catarina - UFSC/BR
/
Universidade Federal de São Paulo - UNIFESP/BR
/
Universidade Federal do Rio Grande do Norte - UFRN/BR
/
Universidade Federal do Rio de Janeiro - UFRJ/BR
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