Detection of Mycobacterium sp. by multiplex PCR directly from suspicious granulomas from cold chambers in the state of Bahia, Brazil
Arq. Inst. Biol
;
88: e00592020, 2021. ilus, tab
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1357869
ABSTRACT
The diagnosis of bovine tuberculosis (TB) by molecular techniques has been broadly studied. These methods allow accelerating the diagnosis, in addition to presenting high specificity and sensitivity in the identification of the pathogen, critical characteristic for public health, especially when it comes to the direct diagnosis of the biologic samples, which has been little explored. This paper has evaluated a multiplex polymerase chain reaction (mPCR) as a tool to diagnose TB, which was performed directly on the granulomatous material of suspicious lesions collected in a cold chamber under state inspection in the state of Bahia, Brazil. Of the 74 samples evaluated, 14.86% were positive, with 10.81% positive for mPCR and culture, 4.05% negative for cultivation and positive for mPCR. The correlation between the cultivation and the mPCR presented agreeance higher than 61.54% of the cases. The results have indicated that the protocol proved itself effective, fast and very promising in the surveillance in slaughterhouses for the diagnosis of tuberculosis directly from the granuloma.(AU)
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tuberculosis Bovina
/
Diagnóstico
/
Reacción en Cadena de la Polimerasa Multiplex
/
Mycobacterium
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
/
Estudio pronóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Arq. Inst. Biol
Año:
2021
Tipo del documento:
Artículo
Institución/País de afiliación:
Universidade Federal do Recôncavo da Bahia/BR
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