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First detection of Leishmania of the subgenus viannia in Alipiopsitta xanthops, endemic bird of South America
Matheus, Larissa Maria Dávalo; Duarte, Pâmella Oliveira; Ferreira, Eduardo de Castro.
  • Matheus, Larissa Maria Dávalo; Federal University of Mato Grosso do Sul. Campo Grande. BR
  • Duarte, Pâmella Oliveira; Federal University of Mato Grosso do Sul. Campo Grande. BR
  • Ferreira, Eduardo de Castro; Oswaldo Cruz Foundation. Campo Grande. BR
Biosci. j. (Online) ; 37: e37014, Jan.-Dec. 2021. ilus
Artículo en Inglés | LILACS | ID: biblio-1358709
ABSTRACT
We report the first molecular detection of Leishmania infection (subgenus Viannia) in the yellow-faced parrot (Alipiopsitta xanthops), at a wildlife rehabilitation center located in the city of Campo Grande, Brazil, an endemic area for leishmaniasis. PCRs targeting kinetoplast DNA (kDNA) and the small subunit of ribosomal RNA of Leishmania spp. were performed, both positive, followed by the sequencing of the amplified region of the SSU rDNA gene, which confirmed the identity of the parasite. This is the first report of success obtained in the use of PCR targeting the IRBP (Interphotoreceptor retinoid-binding protein) gene as an internal control in the molecular diagnosis of pathogens in bird species.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Loros / Leishmaniasis Idioma: Inglés Revista: Biosci. j. (Online) Asunto de la revista: Agricultura / Disciplinas das Ciˆncias Biol¢gicas / Pesquisa Interdisciplinar Año: 2021 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Federal University of Mato Grosso do Sul/BR / Oswaldo Cruz Foundation/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Loros / Leishmaniasis Idioma: Inglés Revista: Biosci. j. (Online) Asunto de la revista: Agricultura / Disciplinas das Ciˆncias Biol¢gicas / Pesquisa Interdisciplinar Año: 2021 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Federal University of Mato Grosso do Sul/BR / Oswaldo Cruz Foundation/BR