Cytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cells
Pesqui. bras. odontopediatria clín. integr
;
22: e210114, 2022. tab, graf
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-1365227
ABSTRACT
ABSTRACT Objective To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods The culture of hPDLSCs was established. Cell density was set at 2 × 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 1:4 and 1:16) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey's test (α=0.05). Results At 24 h, pure extract of MTA Repair HP and Biodentine 1:16 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 1:4 and 1:16, and White MTA 1:16. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Ligamento Periodontal
/
Materiales de Obturación del Conducto Radicular
/
Células Madre
/
Pruebas Inmunológicas de Citotoxicidad
/
Cementos Dentales
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Pesqui. bras. odontopediatria clín. integr
Asunto de la revista:
Odontología
Año:
2022
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
University of São Paulo/BR
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