Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples
Braz. J. Pharm. Sci. (Online)
;
58: e19587, 2022. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1384015
ABSTRACT
This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temperature, flow rate, injection volume and mobile phase with different proportions of solvents were evaluated. The best chromatographic conditions were obtained using the Phenomenex® C18 (150 × 4.60 mm, 5 µm) column at a temperature of 20 °C, flow rate of 1.2 mL min-1, injection volume of 20 µL, and detection at 250 nm. Acetonitrile ultrapure water (87 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and acetonitrile methanol ultrapure water (53 35 12, v/v/v) for abamectin and ivermectin. Under these conditions satisfactory results were obtained, with appropriate limits of detection and quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy the need for analytical methods for the multi-determination of MBMs and the B1a and B1b forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and should aid in the development of the fiel
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Métodos de Análisis de Laboratorio y de Campo
/
Cromatografía Líquida de Alta Presión
/
Estudio de Validación
Idioma:
Inglés
Revista:
Braz. J. Pharm. Sci. (Online)
Asunto de la revista:
Farmacologia
/
Teraputica
/
Toxicologia
Año:
2022
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de São João del-Rei/BR
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