Determination of ATP and its metabolites in dietary energy supplements by capillary electrophoresis
Braz. J. Pharm. Sci. (Online)
;
58: e201045, 2022. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1420433
ABSTRACT
Abstract This study aimed to develop a simple and fast capillary electrophoresis (CE) method for the simultaneous determination of adenosine triphosphate (ATP) and its metabolites in dietary energy supplements. Reverse polarity separation mode for faster separation of the three strong negatively charged analytes and capillaries with a 25 µm inner diameter was employed. At -433 V/cm field strength at background electrolyte (BGE) consist with 0.1 M tris-HCl, 0.5 mM tetradecyltrimethylammonium chloride (TTAC) as positively charged surfactant and 0.3 mg/mL hydroxypropylmethylcellulose (HPMC) to reduce the electroosmotic flow (EOF), a complete separation of the three species were achieved in less than 15 minutes. The data acquisition was conducted at a wavelength of 254 nm. Three different commercialised dietary energy supplements were analysed.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Capilares
/
Adenosina Trifosfato
/
Electroforesis Capilar
Idioma:
Inglés
Revista:
Braz. J. Pharm. Sci. (Online)
Asunto de la revista:
Farmacologia
/
Teraputica
/
Toxicologia
Año:
2022
Tipo del documento:
Artículo
País de afiliación:
Turquía
Institución/País de afiliación:
Inönü University/TR
Similares
MEDLINE
...
LILACS
LIS