Investigation of the histopathological level of Ki-67, caspase-3 expressions of the effects of hesperidin on wound healing in the rat esophagus
Acta cir. bras
;
38: e381723, 2023. tab, ilus
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1429536
ABSTRACT
Purpose:
The effects of hesperidin application on the wound caused by esophageal burns were investigated in this study.Methods:
Wistar albino rats were divided into three groups Control group only 1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn group An alkaline esophageal burn model was created with 0.2 mL of 25% NaOH orally by gavage1 mL of 0.09% NaCl was administered i.p. for 28 days; Burn+Hesperidin group 1 mL of 50 mL/kg of hesperidin was given i.p. for 28 days to rats after burn injury. Blood samples were collected for biochemical analysis. Esophagus samples were processed for histochemical staining and immunohistochemistry.Results:
Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were significantly increased in Burn group. Glutathione (GSH) content and histological scores of epithelialization, collagen formation, neovascularization was decreased. After hesperidin treatment, these values were significantly improved in the Burn+Hesperidin group. In the Burn group, epithelial cells and muscular layers were degenerated. Hesperidin treatment restored these pathologies in Burn+Hesperidin group. Ki-67 and caspase-3 expressions were mainly negative in control group; however, the expression was increased in the Burn group. In the Burn+Hesperidin group, Ki-67 and caspase-3 immune activities were reduced.Conclusion:
Hesperidin dosage and application methods can be developed as an alternative treatment for burn healing and treatment.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Cicatrización de Heridas
/
Apoptosis
/
Antígeno Ki-67
/
Esófago
/
Caspasa 3
/
Hesperidina
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Acta cir. bras
Año:
2023
Tipo del documento:
Artículo
Institución/País de afiliación:
Dicle Üniversitesi/TR
/
Diyarbakir Memorial Hospital/TR
/
Siirt University/TR
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