Your browser doesn't support javascript.
loading
Bioactivity-guided isolation of the antidiabetic principle in Pterocarpus Santalinoides leaf extract
Madubuike, Kelechi Gideon; Nnadi, Charles Okeke; Anaga, Aruh Ottah; Asuzu, Isaac Uzoma.
  • Madubuike, Kelechi Gideon; Michael Okpara University of Agriculture. Department of Veterinary Physiology and Pharmacology. Umudike. NG
  • Nnadi, Charles Okeke; University of Nigeria. Department of Pharmaceutical and Medicinal Chemistry. Nsukka. NG
  • Anaga, Aruh Ottah; University of Nigeria. Department of Veterinary Physiology and Pharmacology. Nsukka. NG
  • Asuzu, Isaac Uzoma; University of Nigeria. Department of Veterinary Physiology and Pharmacology. Nsukka. NG
Braz. J. Pharm. Sci. (Online) ; 59: e21726, 2023. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1439500
ABSTRACT
Abstract Pterocarpus santalinoides is used in Nigerian ethnomedicine to treat diabetes mellitus. This study aimed to establish the antidiabetic property of the plant, and isolate and characterize its active principle. Dried and pulverized leaves (500 g) of P. santalinoides were extracted with 1.8 L of 80 % hydromethanol by cold maceration. The dried extract (10 g) was partitioned into n-hexane, ethyl acetate (EtOAc), n-butanol, and water. Antidiabetic activitiy-guided isolation by column chromatographic separation of the EtOAc soluble and purification of the sub-fractions by repeated preparative thin layer chromatography (pTLC) yielded a C-glycosyl flavonoid, identified as isovitexin. The chemical structure was elucidated based on high-resolution mass spectroscopy, 1D, and 2D nuclear magnetic resonance spectroscopic analyses. Alloxan-induced diabetic rat model was adopted for antidiabetic screening. The extract of P. santalinoides (100-200 mg/kg), fraction F4 (50 mg/kg), sub-fraction F4.3 (10 mg/kg), and the semi-purified compound F4.3.2 (5 mg/kg) significantly (p<0.05) reduced the fasting blood glucose of alloxan-induced diabetic rats, causing 48.4, 69.4, 57.7 and 64.5 % antidiabetic activity respectively, compared with > 68 % recorded in glibenclamide (2 mg/kg) control. These results reveal that isovitexin is the antidiabetic principle in P. santalinoides
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Extractos Vegetales / Pterocarpus / Hipoglucemiantes Límite: Animales Idioma: Inglés Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2023 Tipo del documento: Artículo País de afiliación: Nigeria Institución/País de afiliación: Michael Okpara University of Agriculture/NG / University of Nigeria/NG

Similares

MEDLINE

...
LILACS

LIS


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Extractos Vegetales / Pterocarpus / Hipoglucemiantes Límite: Animales Idioma: Inglés Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2023 Tipo del documento: Artículo País de afiliación: Nigeria Institución/País de afiliación: Michael Okpara University of Agriculture/NG / University of Nigeria/NG