Metabolic study of trimetazidine using ultra-high performance liquid chromatography-tandem mass spectrometry
Braz. J. Pharm. Sci. (Online)
;
59: e22453, 2023. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1439540
ABSTRACT
Abstract In the present study, the application of ultra-high performance liquid chromatography-tandem mass spectrometry allowed us to study of known-as well as hitherto unknown-trimetazidine (TMZ) metabolites in human urine and to propose their renal excretion profiles. Urine samples from a healthy volunteer were analyzed at baseline and at 0-4 h, 4-8 h, 8-12 h, and 12-24 h after a single dose of TMZ. A dilute-and-shoot procedure was used as sample treatment before separation. Full-scan spectra of possible metabolites were acquired. Additionally, product ion scan spectra of precursor ions of interest were also acquired at two collision energies. Intact TMZ was a major excretion product, with a maximum concentration at 4-8 h after administration. Moreover, five minor metabolites were observed, namely trimetazidine-N-oxide (M1), N-formyl trimetazidine (M2), desmethyl-trimetazidine O-sulfate (M3), desmethyl-trimetazidine O-glucuronide (M4), and desmethyl-trimetazidine-N-oxide-O-glucuronide (M5). Metabolite M5 has not previously been reported. Excretion curves were constructed based on the chromatographic peak areas of specific mass transitions (precursor ion > product ion) related to each of the detected metabolites
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Trimetazidina
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masas en Tándem
Límite:
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Braz. J. Pharm. Sci. (Online)
Asunto de la revista:
Farmacologia
/
Teraputica
/
Toxicologia
Año:
2023
Tipo del documento:
Artículo
País de afiliación:
Eslovaquia
/
España
Institución/País de afiliación:
Comenius University/SK
/
Hospital del Mar Institute for Medical Research/ES
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