Effects of Chitosan Nanoparticles with Long Synthetic siRNAs Targeting VEGF in Triple-Negative Breast Cancer Cells
Braz. J. Pharm. Sci. (Online)
;
59: e22304, 2023. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-1447564
ABSTRACT
Abstract Vascular endothelial growth factor (VEGF) is an essential angiogenic factor in breast cancer development and metastasis. Small interfering RNAs (siRNAs) can specifically silence genes via the RNA interference pathway, therefore were investigated as cancer therapeutics. In this study, we investigated the effects of siRNAs longer than 30 base pairs (bp) loaded into chitosan nanoparticles in triple-negative breast cancer cells, compared with conventional siRNAs. 35 bp long synthetic siRNAs inhibited VEGF gene expression by 51.2% and increased apoptosis level by 1.75-fold in MDA-MB-231 cell lines. Furthermore, blank and siRNA-loaded chitosan nanoparticles induced expression of IFN-γ in breast cancer cells. These results suggest that long synthetic siRNAs can be as effective as conventional siRNAs, when introduced into cells with chitosan nanoparticles
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ARN Interferente Pequeño
/
Factor A de Crecimiento Endotelial Vascular
/
Quitosano
/
Nanopartículas
/
Neoplasias de la Mama Triple Negativas
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Braz. J. Pharm. Sci. (Online)
Asunto de la revista:
Farmacologia
/
Teraputica
/
Toxicologia
Año:
2023
Tipo del documento:
Artículo
País de afiliación:
Turquía
Institución/País de afiliación:
Istanbul Medipol University/TR
/
Marmara University/TR
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