Molecular characteristics of polysulfated fractions from the green seaweed Caulerpa cupressoides and actions on thrombin generation in vitro
Acta sci., Biol. sci
;
41: e35655, 20190000. tab, graf
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1460862
ABSTRACT
Caulerpa cupressoides produces sulfated polysaccharides (Cc-SPs) with serpin-dependent anticoagulant effect, but their actions on thrombin generation (TG) are unknown. This study aimed to partially characterize Cc-SPs and examine their potential as modulators of TG. Infrared analysis characterized extract containing three ulvan fractions (Cc-SP1, -SP2 and -SP3) separated by DEAEcellulose chromatography, with differences in the relative proportions of sulfate (10.99-18.38%) and total sugars (46.59-51.12%), without presenting proteins. Charge density patterns and nonSPs varying from 8 to > 100 kDa on agarose and polyacrylamide gel electrophoresis by sequential staining with toluidine blue and stains-all were also confirmed by gel permeation chromatography. The molecular weight of Cc-SP2 was not altered after treatment with 0.4 M HCl up to 5 h. Only Cc-SP2 altered the activated partial thromboplastin time (15 ± 0.3 IU) vs. heparin (193 IU) and abolished at high concentrations (> 4.1 μg) TG by intrinsic pathway in 60-fold diluted human plasma, while at 4.1 μg attenuated TG by 33.87% delaying the lag phase (32 min.) vs. control (28 min.). Cc-SP2 induced concentration-dependent TG in system without cephalin. Heparin abolished TG at 4.15-fold lower amount, but did not stimulate TG. Therefore, Cc-SPs express dual effects on thrombosis in vitro.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Sulfatos
/
Trombina
/
Caulerpa
/
Biología Molecular
Idioma:
Inglés
Revista:
Acta sci., Biol. sci
Año:
2019
Tipo del documento:
Artículo
Institución/País de afiliación:
Universidade Federal do Ceará/BR
/
Universidade Federal do Rio de Janeiro/BR
Similares
MEDLINE
...
LILACS
LIS