Determination of the biodegradability of chitosan utilizing the most probable number technique
Acta sci., Biol. sci
;
42: e52965, fev. 2020. tab, graf, ilus
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1460942
ABSTRACT
The present work aimed to evaluate the degradability of the chitosan polymer by soil microorganisms.This evaluation was accomplished using the Most Probable Number (MPN) method by plating in drops so that soil microorganisms capable of degrading the polymeric material could be quantified. Soil samples diluted in three specific culture media for each typeof microorganism were plated bacteria, fungi and actinobacteriaand they were maintained at 28°C for seven days to determine the growth rate of fungi and actinobacteria, and for 48hoursfor the development of bacteria. Significant differences in the MPN of actinobacteriarelative to the other groups analyzed were observed. Thus, the method used was effective for determining the degradability of the chitosan biopolymer when observing the development of microorganisms subjected to the replacement of thecarbonsource by the addition of 2% w v-1of the chitosan biopolymer to the culture medium. The formation of clear regions around the microbial colonies was a strong indicator of biodegradation.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Biodegradación Ambiental
/
Análisis del Suelo
/
Quitosano
Idioma:
Inglés
Revista:
Acta sci., Biol. sci
Año:
2020
Tipo del documento:
Artículo
Institución/País de afiliación:
Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR
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