Micropropagation and in vitroconservation of Alcantarea nahoumii (Bromeliaceae), an endemic and endangered species of the Brazilian Atlantic Forest
Acta sci., Biol. sci
;
42: e52940, fev. 2020. ilus, tab, graf
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1460951
ABSTRACT
Alcantarea nahoumii(Leme) J. R. Grant is a species native to theAtlantic Forest that stands out for ornamental purposes. The objective of this work was to evaluate the in vitrogerminationof A. nahoumiiseeds and establish a micropropagation protocol for production of seedlings so as to minimize the effects of predatory extractivism and develop an in vitroconservation system. Mature seeds were disinfested, established in three culture media (MS, MS½ and MS⅓) and incubated at four temperatures (20, 25, 30 and 35ºC)in a germination chamber. In the micropropagation experiment, stem segments were introduced in MS medium supplemented with 0.5 μM of 1-naphthaleneacetic acid (NAA) and 0.0, 2.2, 4.4 and 6.6 μM of 6-benzylaminopurine (BAP). For the in vitroconservation, plantlets were established in MS or MS½ medium supplemented with 15 g L-1or 30 g L-1of sucrose. The plants were acclimated with commercial substrate. The highest seed germination percentages were promoted by temperature conditions of 20 and 25ºC, with MS culture medium. The highest multiplication rate of shoots was obtained from the treatment without addition of the growth regulator or when combined with 2.2 μM of BAP + 0.5 μM of NAA. The acclimation of the plants occurred with high survival rate. The species can be conserved in vitrounder slow growth condition for 24 months when incubated in MS medium supplemented with 30 g L-1of sucrose.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Técnicas In Vitro
/
Bosques
/
Conservación de los Recursos Naturales
/
Bromeliaceae
Tipo de estudio:
Guía de Práctica Clínica
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Acta sci., Biol. sci
Año:
2020
Tipo del documento:
Artículo
Institución/País de afiliación:
Embrapa Mandioca e Fruticultura/BR
/
Universidade Federal do Recôncavo da Bahia/BR
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