Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
J. venom. anim. toxins incl. trop. dis
;
20: 1-6, 04/02/2014. tab, ilus
Artículo
en Inglés
| LILACS, VETINDEX
| ID: biblio-1484572
ABSTRACT
Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasites DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasites DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Leishmaniasis
/
Reacción en Cadena de la Polimerasa
/
Diagnóstico
/
Reacciones Falso Negativas
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Año:
2014
Tipo del documento:
Artículo
Institución/País de afiliación:
Centro de Pesquisas Aggeu Magalhães/BR
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