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Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy
Nakutis, Fernanda Serafim; Nishitokukado, Iêda; dos Santos, Fabiana Maria; Ortiz-Agostinho, Carmen Lucia; de Alencar, Daniel Teixeira; Achtschin, Cassiana Ganem; Nunes, Valeria Sutti; Leite, André Zonetti Arruda; Sipahi, Aytan Miranda.
  • Nakutis, Fernanda Serafim; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Nishitokukado, Iêda; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • dos Santos, Fabiana Maria; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Ortiz-Agostinho, Carmen Lucia; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • de Alencar, Daniel Teixeira; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Achtschin, Cassiana Ganem; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Nunes, Valeria Sutti; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Leite, André Zonetti Arruda; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Sipahi, Aytan Miranda; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
Clinics ; 78: 100305, 2023. tab, graf
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-1528419
ABSTRACT
Abstract

Introduction:

Treatments of Inflammatory Bowel Disease (IBD) are able to control symptoms in most cases, however, a fraction of patients do not improve or have a loss of response to treatments, making it important to explore new therapeutic strategies. Hyperbaric oxygen therapy (HBO) may represent one of them. The aim of this study was to evaluate the effects of HBO therapy in an experimental model of IBD.

Methods:

Sixty male BALBc mice were divided into six groups. Group 1 was colitis-induced with trinitrobenzene sulfonic acid (TNBS) + ethanol, group 2 received TNBS + ethanol plus HBO, group 3 received only ethanol, group 4 received ethanol plus HBO, group 5 received saline solution, and group 6 received saline solution plus HBO. HBO was performed for four days, subsequently, the mice were evaluated daily. At the end of the study, samples from the intestine were collected for histological analysis as well as for measurement of antioxidant enzymes and cytokine levels.

Results:

HBO significantly improved the clinical and histological status of the animals. Treatment with HBO increased the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in all of the groups; moreover, the difference was only significant between the TNBS and TNBS + HBO groups and treatments promoted a reduction in the proinflammatory cytokines IFN-γ, IL-12, IL-17 and TNF-α and increased the anti-inflammatory cytokines IL-4 and IL-10, with no changes in IL-13.

Conclusion:

HBO effectively treats TNBS-induced colitis by increasing the activity of antioxidant enzymes and modulating cytokine profiles.


Texto completo: Disponible Índice: LILACS (Américas) Idioma: Inglés Revista: Clinics Asunto de la revista: Medicina Año: 2023 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade de São Paulo/BR

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Texto completo: Disponible Índice: LILACS (Américas) Idioma: Inglés Revista: Clinics Asunto de la revista: Medicina Año: 2023 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade de São Paulo/BR