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Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice
Morello, Ricardo José; Koike, Marcia Kiyomi; Abrahão, Marcos de Souza; Saad, Karen Ruggeri; Saad, Paulo Fernandes; Montero, Edna Frasson de Souza.
  • Morello, Ricardo José; Universidade Federal de São Paulo. Medical School. Operative Technique and Experimental Surgery Division. BR
  • Koike, Marcia Kiyomi; Universidade Federal de São Paulo. Medical School. Operative Technique and Experimental Surgery Division. BR
  • Abrahão, Marcos de Souza; Universidade Federal de São Paulo. Medical School. Operative Technique and Experimental Surgery Division. BR
  • Saad, Karen Ruggeri; Universidade Federal de São Paulo. Medical School. Operative Technique and Experimental Surgery Division. BR
  • Saad, Paulo Fernandes; Universidade Federal de São Paulo. Medical School. Operative Technique and Experimental Surgery Division. BR
  • Montero, Edna Frasson de Souza; Universidade Federal de São Paulo. Medical School. Operative Technique and Experimental Surgery Division. BR
Acta cir. bras ; 31(10): 675-679, Oct. 2016. graf
Artículo en Inglés | LILACS | ID: biblio-827651
ABSTRACT
ABSTRACT

PURPOSE:

To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model.

METHODS:

Two groups were constituted The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically.

RESULTS:

The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx (5.2±0.4 vs 9.0±0.8) and IPC-R-Fk increased the graft development compared with IPC-R (11.2±0.7 and 10.2±0.8). In ALO group, IPC-Fk increased the development compared with ALO-Tx and ALO with IPC-R (6.0±0.8, 9.0±1.2, 0.0±0.0, 0.5±0.3). The PCNA expression was increased in ISO group treated with Fk and IPC-R compared to other groups (12.2±0.8 vs Tx 8.8±0.9, IPC-R 8.0±0.4 and Fk 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx.

CONCLUSION:

Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Trasplante de Tejido Fetal / Tacrolimus / Precondicionamiento Isquémico / Inmunosupresores / Intestino Delgado Tipo de estudio: Estudios de evaluación / Estudio pronóstico Límite: Animales Idioma: Inglés Revista: Acta cir. bras Asunto de la revista: Cirugía General / Procedimentos Cir£rgicos Operat¢rios Año: 2016 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Federal de São Paulo/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Trasplante de Tejido Fetal / Tacrolimus / Precondicionamiento Isquémico / Inmunosupresores / Intestino Delgado Tipo de estudio: Estudios de evaluación / Estudio pronóstico Límite: Animales Idioma: Inglés Revista: Acta cir. bras Asunto de la revista: Cirugía General / Procedimentos Cir£rgicos Operat¢rios Año: 2016 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Federal de São Paulo/BR