Hepatotoxicity and nephrotoxicity of 3-bromopyruvate in mice
Acta cir. bras
;
31(11): 724-729, Nov. 2016. graf
Artículo
en Inglés
| LILACS
| ID: biblio-827660
ABSTRACT
ABSTRACT PURPOSE:
To investigate the hepatotoxicity and nephrotoxicity of 3-Bromopyruvate (3BP) in mice.METHODS:
Fifteen nude mice were grafted subcutaneously in the left flank with MDA-MB-231 cells, then all mice were divided into control group (PBS), 3BP group (8 mg/kg), positive group (DNR 0.8 mg/kg) when tumor volume reached approximately 100 mm3. 28 days later, tumors, livers and kidneys were stored in 4 % formalin solution and stained with hematoxylin and eosin staining. The Kunming mice experiment included control group (PBS), 3BP group (4mg/kg; 8mg/kg; 16mg/kg), positive group (DNR 0.8 mg/kg). 24 hours later, the blood were used for the determination of hepatic damage serum biomarkers. Livers were stored in 4 % formalin solution for the later detection.RESULTS:
3BP at the dose of 8mg/kg had a good effect on inhibiting tumor growth in nude mice and did not damage liver and kidney tissues. Kunming mice experiment showed 3BP at the dose of 16mg/kg did damage to liver tissues.CONCLUSION:
3-Bromopyruvate at the dose of suppressing tumor growth did not exhibit hepatotoxicity and nephrotoxicity in nude mice, and the effect on liver was confirmed in Kunming mice.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Piruvatos
/
Inhibidores Enzimáticos
/
Enfermedad Hepática Inducida por Sustancias y Drogas
/
Lesión Renal Aguda
/
Riñón
/
Hígado
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Department of Pharmacy/CN
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