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Grandisin induces apoptosis in leukemic K562 cells
Cortez, Alane Pereira; Menezes, Elizabeth Gomes Paulino; Benfica, Polyana Lopes; Santos, Alexandre Pereira dos; Cleres, Larissa Moreira; Ribeiro, Higor de Oliveira; Lima, Eliana Martins; Kato, Massuo Jorge; Valadares, Marize Campos.
  • Cortez, Alane Pereira; Federal University of Goiás. Faculty of Pharmacy. Laboratory of Celullar Toxicology and Pharmacology - FarmaTec. Goiânia. BR
  • Menezes, Elizabeth Gomes Paulino; Federal University of Goiás. Faculty of Pharmacy. Laboratory of Celullar Toxicology and Pharmacology - FarmaTec. Goiânia. BR
  • Benfica, Polyana Lopes; Federal University of Goiás. Faculty of Pharmacy. Laboratory of Celullar Toxicology and Pharmacology - FarmaTec. Goiânia. BR
  • Santos, Alexandre Pereira dos; Federal University of Goiás. Faculty of Pharmacy. Laboratory of Celullar Toxicology and Pharmacology - FarmaTec. Goiânia. BR
  • Cleres, Larissa Moreira; Federal University of Goiás. Faculty of Pharmacy. Laboratory of Celullar Toxicology and Pharmacology - FarmaTec. Goiânia. BR
  • Ribeiro, Higor de Oliveira; Federal University of Goiás. Faculty of Pharmacy. Laboratory of Celullar Toxicology and Pharmacology - FarmaTec. Goiânia. BR
  • Lima, Eliana Martins; Federal University of Goiás. Faculty of Pharmacy. Medicinal Pharmaceutical Chemistry Laboratory. Goiânia. BR
  • Kato, Massuo Jorge; University of São Paulo. Institute of Chemistry. Chemistry Laboratory of Natural Products. São Paulo. BR
  • Valadares, Marize Campos; Federal University of Goiás. Faculty of Pharmacy. Laboratory of Celullar Toxicology and Pharmacology - FarmaTec. Goiânia. BR
Braz. J. Pharm. Sci. (Online) ; 53(1): e15210, 2017. graf
Artículo en Inglés | LILACS | ID: biblio-839446
ABSTRACT
Abstract In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85µM. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was confirmed by anexin V stain and caspase activation. Thus, lignan grandisin showed antileukemic activities against the K562 cell line and the cell death process occurred via apoptosis.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Regulación Leucémica de la Expresión Génica / Lignanos / Células K562 / Factor Inductor de la Apoptosis Idioma: Inglés Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2017 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Federal University of Goiás/BR / University of São Paulo/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Regulación Leucémica de la Expresión Génica / Lignanos / Células K562 / Factor Inductor de la Apoptosis Idioma: Inglés Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2017 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Federal University of Goiás/BR / University of São Paulo/BR