Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
J. appl. oral sci
;
24(6): 575-581, Nov.-Dec. 2016. graf
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-841149
ABSTRACT
ABSTRACT Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05). Conclusion The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tratamiento del Conducto Radicular
/
Terapia por Ultrasonido
/
Hidróxido de Calcio
/
Enterococcus faecalis
/
Cavidad Pulpar
/
Dentina
/
Antiinfecciosos Locales
Tipo de estudio:
Estudios de evaluación
Límite:
Animales
Idioma:
Inglés
Revista:
J. appl. oral sci
Asunto de la revista:
Odontología
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de São Paulo/BR
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