Comparison of two different methods for detecting periodontal pathogenic bacteria
Braz. j. oral sci
;
15(3)July-Sept. 2016. ilus
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-875020
ABSTRACT
Aim:
To perform a comparative analysis between two methods for detecting Porphyromonas gingivalis, Tannerella forsythia and Porphyromonas endodontalis in periodontal plaque samples.Methods:
The study sample consisted of twenty systemically healthy patients showing generalized chronic periodontitis. The subgingival samples for microbiological analysis were collected before (baseline) and 60 days after a basic periodontal therapy from 30 non-adjacent affected sites (Probing Depth (PD) 5-7 mm, Clinical Attachment Loss (CAL) ≥ 5 mm, positive for Bleeding on Probing (BOP)). Microbiological analysis was performed by PCR and qPCR. To allow a comparative analysis between both methods, qPCR was divided in three different scores (score 2 presence of more than 100 bacteria; score 1 presence of 10-100 bacteria, and score 0 absence of bacteria), in accordance to DNA quantity, while for PCR two scores were assigned presence or absence of bacteria.Results:
qPCR demonstrated higher sensitivity in the detection of these pathogens compared with PCR when scores 1 and 2 were considered positive. However, when only score 2 was considered positive, PCR and qPCR showed better agreement.Conclusions:
qPCR demonstrated higher sensitivity than conventional PCR for detection of low numbers of microorganisms and can be useful for the quantification of periodontopathogens. (AU)
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Enfermedades Periodontales
/
Bacterias
/
Reacción en Cadena de la Polimerasa
/
Periodontitis Crónica
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Braz. j. oral sci
Asunto de la revista:
Odontología
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Nove de Julho University/BR
/
State University of São Paulo/BR
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