Your browser doesn't support javascript.
loading
The Role of Histone Deacetylase Inhibitors in Regulation of Akt/GSK-3ß Signaling Pathway in Mice Following Transient Focal Cerebral Ischemia
Zhao, Bo; Liu, Lian; Leng, Yan; Yuan, Quan; Hou, Jiabao; Wu, Yang; Gao, Wenwei.
  • Zhao, Bo; Wuhan University. Renmin Hospital. Department of Anesthesiology. CN
  • Liu, Lian; Wuhan University. Renmin Hospital. Department of Anesthesiology. CN
  • Leng, Yan; Wuhan University. Renmin Hospital. Department of Anesthesiology. CN
  • Yuan, Quan; Wuhan University. Renmin Hospital. Department of Anesthesiology. CN
  • Hou, Jiabao; Wuhan University. Renmin Hospital. Department of Anesthesiology. CN
  • Wu, Yang; Wuhan University. Renmin Hospital. Department of Anesthesiology. CN
  • Gao, Wenwei; Wuhan University. Renmin Hospital. Department of Anesthesiology. CN
Acta cir. bras ; 32(10): 862-872, Oct. 2017. graf
Artículo en Inglés | LILACS | ID: biblio-886174
ABSTRACT
Abstract

Purpose:

To investigate whether the neuroprotective effect of TSA on cerebral ischemia reperfusion injury is mediated by the activation of Akt/GSK-3β signaling pathway.

Methods:

Mice were randomly divided into four groups (n=15) sham group (S); ischemia reperfusion group (IR); ischemia reperfusion and pretreated with TSA group (IR+T); ischemia reperfusion and pretreated with TSA and LY294002 group (IR+T+L). The model of cerebral ischemia reperfusion was established by 1h of MCAO following 24h of reperfusion. TSA (5mg/kg) was intraperitoneally given for 3 days before MCAO, Akt inhibitor, LY294002 (15 nmol/kg) was injected by tail vein 30 min before the MCAO.

Results:

TSA significantly increased the expression of p-Akt, p-GSK-3β proteins and the levels of SOD, Bcl-2, reduced the infarct volume and the levels of MDA, ROS, TNF-α, IL-1β, Bax, Caspase-3, TUNEL and attenuated neurological deficit in mice with transient MCAO, LY294002 weakened such effect of TSA dramatically.

Conclusions:

TSA could significantly decrease the neurological deficit and reduce the cerebral infarct volume, oxidative stress, inflammation, as well as apoptosis during cerebral ischemia reperfusion injury, which was achieved by activation of the Akt/GSK-3β signaling pathway.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Transducción de Señal / Ataque Isquémico Transitorio / Fármacos Neuroprotectores / Glucógeno Sintasa Quinasa 3 / Proteínas Proto-Oncogénicas c-akt / Inhibidores de Histona Desacetilasas Límite: Animales Idioma: Inglés Revista: Acta cir. bras Asunto de la revista: Cirugía General / Procedimentos Cir£rgicos Operat¢rios Año: 2017 Tipo del documento: Artículo País de afiliación: China Institución/País de afiliación: Wuhan University/CN

Similares

MEDLINE

...
LILACS

LIS


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Transducción de Señal / Ataque Isquémico Transitorio / Fármacos Neuroprotectores / Glucógeno Sintasa Quinasa 3 / Proteínas Proto-Oncogénicas c-akt / Inhibidores de Histona Desacetilasas Límite: Animales Idioma: Inglés Revista: Acta cir. bras Asunto de la revista: Cirugía General / Procedimentos Cir£rgicos Operat¢rios Año: 2017 Tipo del documento: Artículo País de afiliación: China Institución/País de afiliación: Wuhan University/CN