Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
Acta cir. bras
;
32(8): 607-616, Aug. 2017. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-886227
ABSTRACT
Abstract Purpose:
To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models.Methods:
Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (31). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer's lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed.Results:
Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups.Conclusions:
The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Cauterización
/
Neovascularización de la Córnea
/
Compuestos de Potasio
/
Modelos Animales de Enfermedad
/
Neovascularización Patológica
/
Nitratos
Tipo de estudio:
Estudios de evaluación
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2017
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Esatdual de São Paulo/BR
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