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Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei, Mytilidae) in environmental samples
PIE, MARCIO R; STRÖHER, PATRÍCIA R; AGOSTINIS, ANDRÉ O; BELMONTE-LOPES, RICARDO; TADRA-SFEIR, MICHELLE Z; OSTRENSKY, ANTONIO.
Afiliación
  • PIE, MARCIO R; Universidade Federal do Paraná. Departamento de Zoologia. Curitiba. BR
  • STRÖHER, PATRÍCIA R; Universidade Federal do Paraná. Departamento de Zoologia. Curitiba. BR
  • AGOSTINIS, ANDRÉ O; Universidade Federal do Paraná. Departamento de Zoologia. Curitiba. BR
  • BELMONTE-LOPES, RICARDO; Universidade Federal do Paraná. Departamento de Zoologia. Curitiba. BR
  • TADRA-SFEIR, MICHELLE Z; Universidade Federal do Paraná. Departamento de Zoologia. Curitiba. BR
  • OSTRENSKY, ANTONIO; Universidade Federal do Paraná. Departamento de Zoologia. Curitiba. BR
An. acad. bras. ciênc ; 89(2): 1041-1045, Apr.-June 2017. tab, graf
Article en En | LILACS | ID: biblio-886687
Biblioteca responsable: BR1.1
ABSTRACT
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.
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Texto completo: 1 Índice: LILACS Asunto principal: Mytilidae / Especies Introducidas / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: An. acad. bras. ciênc Asunto de la revista: CIENCIA Año: 2017 Tipo del documento: Article

Texto completo: 1 Índice: LILACS Asunto principal: Mytilidae / Especies Introducidas / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: An. acad. bras. ciênc Asunto de la revista: CIENCIA Año: 2017 Tipo del documento: Article