Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
An. acad. bras. ciênc
;
90(1): 99-108, Mar. 2018. graf
Artículo
en Inglés
| LILACS
| ID: biblio-886876
ABSTRACT
ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Compuestos de Sulfhidrilo
/
Cisteamina
/
Cistina
/
Disulfuros
/
Homeostasis
/
Riñón
Tipo de estudio:
Estudios de evaluación
Límite:
Animales
Idioma:
Inglés
Revista:
An. acad. bras. ciênc
Asunto de la revista:
Ciencia
Año:
2018
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal do Rio Grande do Sul/BR
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