Effects of drying and storage conditions on the stability of TSH in blood spots
Arch. endocrinol. metab. (Online)
;
62(2): 201-204, Mar.-Apr. 2018. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-887641
ABSTRACT
ABSTRACT Objective To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening. Subjects and methods Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage. Results The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer. Conclusions Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tirotropina
/
Recolección de Muestras de Sangre
/
Tamizaje Neonatal
/
Liofilización
Tipo de estudio:
Estudios de evaluación
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
/
Recién Nacido
Idioma:
Inglés
Revista:
Arch. endocrinol. metab. (Online)
Asunto de la revista:
Endocrinologia
/
Metabolismo
Año:
2018
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de São Paulo/BR
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