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Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells
Wang, WJ; Cheng, MH; Lin, JH; Weng, CS.
  • Wang, WJ; Chung Yuan Christian University. Department of Biomedical Engineering. Chungli. TW
  • Cheng, MH; Chung Yuan Christian University. Department of Biomedical Engineering. Chungli. TW
  • Lin, JH; Chung Yuan Christian University. Department of Biomedical Engineering. Chungli. TW
  • Weng, CS; Chung Yuan Christian University. Department of Biomedical Engineering. Chungli. TW
Braz. j. med. biol. res ; 50(12): e6145, 2017. tab, graf
Artículo en Inglés | LILACS | ID: biblio-888968
ABSTRACT
Chronic systemic inflammation and repetitive damage of vascular endothelia by incompatible dialysis system are probable causes of cardiovascular disease in patients on dialysis. The present study aimed to assess in vitro biocompatibility and anti-inflammatory effect of hemodialysis fluid supplemented with rosmarinic acid (RA) using human umbilical vein endothelial cells (HUVEC). HUVECs (5×106 cells/mL) were pre-exposed to 1 μg/mL of lipopolysaccharides (LPS) and incubated with RA-supplemented hemodialysis fluid (HDF). Cytotoxicity was assessed qualitatively by morphologic assessment and quantitatively by MTT assay. Expressions of proinflammatory mediators were assessed using quantitative real-time PCR and production of NO was quantified. Phosphorylation of AKT and nuclear localization of nuclear factor kappa B (NF-κB) were examined using western blotting. Exposure of HUVECs to RA-supplemented HDF had no influence on morphology and viability. Inhibition of proinflammatory mediator production in HUVECs by RA supplementation to HDF was significant in a dose-dependent manner. Exposure to RA-supplemented HDF resulted in a decrease in nitric oxide synthase expression and reduction of NO production in LPS-stimulated HUVECs. RA supplementation of HDF suppressed Akt activation in LPS-stimulated HUVECs. In addition, the level of cellular IκB was increased in parallel to a reduced nuclear translocation of NF-κB in LPS-induced endothelial cells. Our results suggest that RA-supplemented HDF is biocompatible and significantly suppressed inflammation induced in endothelial cells. In this respect, the use of HDF supplemented with RA could alleviate inflammation and improve long-term treatment of patients with renal failure on dialysis. Further clinical studies are required to confirm the effects.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Materiales Biocompatibles / Soluciones para Hemodiálisis / Antiinflamatorios no Esteroideos / Cinamatos / Depsidos / Células Endoteliales de la Vena Umbilical Humana / Inflamación Tipo de estudio: Estudios de evaluación Límite: Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2017 Tipo del documento: Artículo País de afiliación: Taiwán Institución/País de afiliación: Chung Yuan Christian University/TW

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Materiales Biocompatibles / Soluciones para Hemodiálisis / Antiinflamatorios no Esteroideos / Cinamatos / Depsidos / Células Endoteliales de la Vena Umbilical Humana / Inflamación Tipo de estudio: Estudios de evaluación Límite: Humanos Idioma: Inglés Revista: Braz. j. med. biol. res Asunto de la revista: Biologia / Medicina Año: 2017 Tipo del documento: Artículo País de afiliación: Taiwán Institución/País de afiliación: Chung Yuan Christian University/TW