HPLC method development and validation for the estimation of Axitinib in rabbit plasma
Braz. J. Pharm. Sci. (Online)
;
53(3): e00012, 2017. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-889392
ABSTRACT
ABSTRACT A rapid, sensitive, and accurate high performance liquid chromatography for the determination of Axitinib (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinib is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 6535 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic runtime is 10.0 min with a retention time for Axitinib and IS of 5.685, and 3.606 min, respectively. The method was validated over a dynamic linear range of 0.002-0.2µg/mL for Axitinib with a correlation coefficient of r2 0.999.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Estudio de Validación
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. J. Pharm. Sci. (Online)
Asunto de la revista:
Farmacologia
/
Teraputica
/
Toxicologia
Año:
2017
Tipo del documento:
Artículo
País de afiliación:
India
Institución/País de afiliación:
Hindu College of Pharmacy/IN
/
V.V. Institute of Pharmaceutical Sciences/IN
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