Development and validation of stability indicating liquid chromatographic (RP-HPLC) method for estimation of ubidecarenone in bulk drug and formulations using quality by design (QBD) approach
Braz. J. Pharm. Sci. (Online)
;
53(4): e17293, 2017. tab, graf, ilus
Artículo
en Inglés
| LILACS
| ID: biblio-889435
ABSTRACT
ABSTRACT A novel, accurate, precise and economical stability indicating Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method, was developed and validated for the quantitative determination of ubidecarenone (UDC) in bulk drug, UDC marketed formulation and UDC loaded cubosomes (CBMs) nanocarriers through Response surface methodology (RSM) design with three factors and three levels was performed to optimize the chromatographic variables followed by forced degradation studies of UDC were performed to detect degradation peak. RP-HPLC separation was achieved using mobile phase consisting of AcetonitrileTetrahydrofuranDeionised water in the ratio 55423 and a flow rate of 1.0 mL/min was optimized with a standard retention time (Rt) of 2.15 min, through experiment. The method was found linear in the concentration range of 5-100 µg/mL with a regression coefficient of 0.999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 3.04 µg/mL and 9.11 µg/mL, respectively.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Ubiquinona
/
Cromatografía de Fase Inversa
Idioma:
Inglés
Revista:
Braz. J. Pharm. Sci. (Online)
Asunto de la revista:
Farmacologia
/
Teraputica
/
Toxicologia
Año:
2017
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
India
/
Arabia Saudita
Institución/País de afiliación:
Glocal University/IN
/
King Saud University/SA
/
School of Pharmaceutical Education & Research/IN
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