Isolation and identification of cytotoxic compounds from a fruticose lichen Roccella montagnei, and it's in silico docking study against CDK-10
Rev. bras. farmacogn
;
27(6): 724-728, Nov.-Dec. 2017. graf
Artículo
en Inglés
| LILACS
| ID: biblio-898721
ABSTRACT
ABSTRACT Roccella montagnei Bél. belongs to lichen family Roccelleceae growing luxuriantly along the coastal regions of India. As Roccella has been shown to be bioactive, we prepared methanolic extract and assessed its anticancer potential. The methanolic extract showed significant in vitro cytotoxic activity against four human cancer cell lines such as colon (DLD-1, SW-620), breast (MCF-7), head and neck (FaDu). This prompted us to isolate bioactive compounds through column chromatography. Two compounds roccellic acid and everninic acid have been isolated, out of which everninic acid is reported for the first time. Both the compounds have been tested for in vitro cytotoxic activity in which roccellic acid showed strong anticancer activity as compared to the everninic acid. Cyclin Dependent Kinase (CDK-10) contributes to proliferation of cancer cells, and aberrant activity of these kinases has been reported in a wide variety of human cancers. These kinases therefore constitute biomarkers of proliferation and attractive pharmacological targets for development of anticancer therapeutics. Therefore both the isolated compounds were tested for in silico molecular docking study against Cyclin Dependent Kinase isomer enzyme to support the cytotoxic activity.
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Índice:
LILACS (Américas)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Rev. bras. farmacogn
Asunto de la revista:
Farmacia
Año:
2017
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
India
Institución/País de afiliación:
CSIR-National Botanical Research Institute/IN
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