Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
Biol. Res
;
47: 1-9, 2014. ilus, graf
Artículo
en Inglés
| LILACS
| ID: biblio-950729
ABSTRACT
BACKGROUND: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential. RESULTS: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude. CONCLUSIONS: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Fotoquimioterapia
/
Lesiones Precancerosas
/
Neoplasias de la Boca
/
Queratinocitos
/
Apoptosis
/
Análisis por Matrices de Proteínas
Límite:
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2014
Tipo del documento:
Artículo
País de afiliación:
Rumanía
Institución/País de afiliación:
National Institute for Research & Development in Chemistry and Petrochemistry/RO
/
National Institute of Pathology/RO
/
University of Medicine and Pharmacy/RO
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