Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
Biol. Res
;
47: 1-6, 2014. ilus
Artículo
en Inglés
| LILACS
| ID: biblio-950748
ABSTRACT
BACKGROUND:
Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.RESULTS:
Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.CONCLUSIONS:
Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Epitelio Seminífero
/
Neoplasias Testiculares
/
Seminoma
/
Proteínas de la Membrana
Tipo de estudio:
Estudio pronóstico
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2014
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Fifth People's Hospital of Chengdu/CN
/
Sichuan Universit/CN
Similares
MEDLINE
...
LILACS
LIS