Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
Biol. Res
;
47: 1-5, 2014. ilus, graf
Artículo
en Inglés
| LILACS
| ID: biblio-950771
ABSTRACT
BACKGROUND: BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells. RESULTS: The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the combined detection also demonstrated the same trend as that obtained from the separate detection, and dosages of 200, 400 and 800 ng/ml testing reagent significantly inhibited the proliferation of rat airway smooth muscle cells. CONCLUSIONS: Our study suggests that the BrdU and WST-1 measurements can be applied simultaneously without mutual interference, which may increase the efficacy and consistency of these measurements to a certain extent.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Evaluación de la Tecnología Biomédica
/
Sales de Tetrazolio
/
Tráquea
/
Bromodesoxiuridina
/
Miocitos del Músculo Liso
/
Proliferación Celular
Tipo de estudio:
Estudio diagnóstico
/
Evaluación de Tecnologías en Salud
Límite:
Animales
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2014
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Shanghai University of Traditional Chinese Medicine/CN
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