Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
Biol. Res
;
48: 1-6, 2015. ilus, tab
Artículo
en Inglés
| LILACS
| ID: biblio-950814
ABSTRACT
BACKGROUND:
DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz. DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world.RESULTS:
Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively.CONCLUSIONS:
Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Neoplasias Colorrectales
/
Genes Supresores de Tumor
/
Metilación de ADN
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2015
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Amazon State University/BR
/
Carlos Chagas Institute/BR
/
FIOCRUZ/BR
/
Federal University of Amazonas/BR
/
Federal University of Rio de Janeiro/BR
/
Oncology Control Center Foundation of Amazon State/BR
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