Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer
Biol. Res
;
49: 1-8, 2016. ilus, graf
Artículo
en Inglés
| LILACS
| ID: biblio-950853
ABSTRACT
BACKGROUND: Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer. METHODS: Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expression in human pancreatic tumors. Then short hairpin RNA (shRNA) targeting ZFR was designed and delivered into PANC-1 pancreatic cancer cells to knock down ZFR expression. Cell viability, cell proliferation and cell cycle analysis after ZFR knockdown were determined by MTT, colony forming and FACS, respectively. In addition, cell migration and invasion were assessed using the Transwell system. RESULTS: The expression of ZFR was significantly higher in pancreatic tumors than normal pancreas tissues by Oncomine database analysis. Knockdown of ZFR by shRNA-expressing lentivirus significantly decreased the viability and invasion ability of pancreatic cancer cells. Moreover, FACS analysis showed that knockdown of ZFR in PANC-1 cells caused a significant cell cycle arrest at G0/G1 phase. Furthermore, knockdown of ZFR decreased the levels of CDK2, CDK4, CyclinA and CyclinD1 and enhanced the expression of p27, which has evidenced by qRT-PCR and Western blot analysis. CONCLUSIONS: Knockdown of ZFR might provide a novel alternative to targeted therapy of pancreatic cancer and deserves further investigation.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Neoplasias Pancreáticas
/
Proteínas de Unión al ARN
/
ARN Interferente Pequeño
/
Técnicas de Silenciamiento del Gen
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Chengdu Medical College/CN
/
Chengdu Military General Hospital/CN
/
Medical Universit/CN
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