Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon)
Biol. Res
;
49: 1-12, 2016. ilus, graf, tab
Artículo
en Inglés
| LILACS
| ID: biblio-950870
ABSTRACT
BACKGROUND:
The olfactomedin-like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. However, no expression of their orthologous has ever been reported in human and baboon.OBJECTIVE:
The aim of this study was to investigate the expression of OLFML2A and OLFML2B in ocular tissues of baboons (Papio hamadryas) and humans, as a key to elucidate OLFML function in eye physiology.METHODS:
OLFML2A and OLFML2B cDNA detection in ocular tissues of these species was performed by RT-PCR. The amplicons were cloned and sequenced, phylogenetically analyzed and their proteins products were confirmed by immunofluorescence assays.RESULTS:
OLFML2A and OLFML2B transcripts were found in human cornea, lens and retina and in baboon cornea, lens, iris and retina. The baboon OLFML2A and OLFML2B ORF sequences have 96% similarity with their human's orthologous. OLFML2A and OLFML2B evolution fits the hypothesis of purifying selection. Phylogenetic analysis shows clear orthology in OLFML2A genes, while OLFML2B orthology is not clear.CONCLUSIONS:
Expression of OLFML2A and OLFML2B in human and baboon ocular tissues, including their high similarity, make the baboon a powerful model to deduce the physiological and/or metabolic function of these proteins in the eye.
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LILACS (Américas)
Asunto principal:
Glicoproteínas
/
Proteínas de la Matriz Extracelular
/
Ojo
/
Proteínas de la Membrana
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
México
Institución/País de afiliación:
Fundación de Asistencia Privada Conde de Valenciana/MX
/
Universidad Autónoma de Guadalajara/MX
/
Universidad Autónoma de Nuevo León/MX
/
Universidad Autónoma de Zacatecas/MX
/
Universidad Nacional Autónoma de México/MX
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