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miR-22 suppresses tumorigenesis and improves radiosensitivity of breast cancer cells by targeting Sirt1
Zhang, Xia; Li, Yuehua; Wang, Dan; Wei, Xiaoer.
  • Zhang, Xia; Shanghai Jiao Tong University. Sixth People's Hospital. Department of Diagnostic and Interventional Radiology. Shanghai. CN
  • Li, Yuehua; Shanghai Jiao Tong University. Sixth People's Hospital. Department of Diagnostic and Interventional Radiology. Shanghai. CN
  • Wang, Dan; Shanghai Jiao Tong University. Sixth People's Hospital. Department of Diagnostic and Interventional Radiology. Shanghai. CN
  • Wei, Xiaoer; Shanghai Jiao Tong University. Sixth People's Hospital. Department of Diagnostic and Interventional Radiology. Shanghai. CN
Biol. Res ; 50: 27, 2017. graf
Artículo en Inglés | LILACS | ID: biblio-950878
ABSTRACT

BACKGROUND:

miR-22 has been shown to be frequently downregulated and act as a tumor suppressor in multiple cancers including breast cancers. However, the role of miR-22 in regulating the radioresistance of breast cancer cells, as well as its underlying mechanism is still not well understood.

METHODS:

The expressions of miR-22 and sirt1 at mRNA and protein levels were examined by qRT-PCR and Western Blot. The effects of miR-22 overexpression and sirt1 knockdown on cell viability, apoptosis, radiosensitivity, γ-H2AX foci formation were evaluated by CCK-8 assay, flow cytometry, colony formation assay, and γ-H2AX foci formation assay, respectively. Luciferase reporter assay and qRT-PCR analysis were performed to confirm the interaction between miR-22 and sirt1.

RESULTS:

miR-22 was downregulated and sirt1 was upregulated at both mRNA and protein levels in breast cancer cells. miR-22 overexpression or sirt1 knockdown significantly suppressed viability, induced apoptosis, reduced survival fraction, and increased the number of γ-H2AX foci in breast cancer cells. Sirt1 was identified as a target of miR-22 and miR-22 negatively regulated sirt1 expression. Ectopic expression of sirt1 dramatically reversed the inhibitory effect of miR-22 on cell viability and promotive effect on apoptotic rates and radiosensitivity in breast cancer cells.

CONCLUSIONS:

miR-22 suppresses tumorigenesis and improves radiosensitivity of breast cancer cells by targeting sirt1, providing a promising therapeutic target for breast cancer.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Tolerancia a Radiación / Neoplasias de la Mama / MicroARNs / Sirtuina 1 Límite: Femenino / Humanos Idioma: Inglés Revista: Biol. Res Asunto de la revista: Biologia Año: 2017 Tipo del documento: Artículo País de afiliación: China Institución/País de afiliación: Shanghai Jiao Tong University/CN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Tolerancia a Radiación / Neoplasias de la Mama / MicroARNs / Sirtuina 1 Límite: Femenino / Humanos Idioma: Inglés Revista: Biol. Res Asunto de la revista: Biologia Año: 2017 Tipo del documento: Artículo País de afiliación: China Institución/País de afiliación: Shanghai Jiao Tong University/CN