CMPD1 inhibited human gastric cancer cell proliferation by inducing apoptosis and G2/M cell cycle arrest
Biol. Res
;
51: 11, 2018. graf
Artículo
en Inglés
| LILACS
| ID: biblio-950897
ABSTRACT
BACKGROUND:
Gastric cancer occupies the fourth highest morbidity rate of cancers worldwide. Clinical therapies of gastric cancer remain limited because of uncertainty of mechanisms and shortness of effective medicine. Thus, new drug candidates for gastric cancer treatment is urgently needed.RESULTS:
In this study, CMPD1 as a wildly used MK2 phosphorylation inhibitor was employed to find its impact on gastric cancer cell proliferation, apoptosis and cell cycle using colony formation assay and flow cytometry analysis. Along with its anti-proliferation effect on gastric cancer cell line MKN-45 and SGC7901, CMPD1 also induced massive apoptosis and significant G2/M phase arrest in a time-dependent and dose-dependent manner in MKN-45 cells respectively. Furthermore, Western blot confirmed that the expression of anti-apoptotic proteins Bcl-2 was decreased while BAX, cytochrome c release and cleaved PARP were increased. In addition, oncogene c-Myc was downregulated in response to CMPD1 treatment.CONCLUSIONS:
Our results demonstrated that CMPD1 has anti-tumor effect on human gastric cancer cell line MKN- 45 possibly via downregulating oncogene c-Myc expression and CMPD1 could be applied as a potential candidate for treating gastric malignancy. To the best of our knowledge, it is the first report of anti-tumor effect of CMPD-1 on human gastric cancer cells.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Neoplasias Gástricas
/
Proteínas Serina-Treonina Quinasas
/
Apoptosis
/
Péptidos y Proteínas de Señalización Intracelular
/
Proliferación Celular
/
Factor de Transcripción SOX9
/
Puntos de Control de la Fase G2 del Ciclo Celular
/
Antineoplásicos
Tipo de estudio:
Estudios de evaluación
Límite:
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2018
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Shanghai Jiao Tong University/CN
/
The First Affiliated Hospital of Bengbu Medical College/CN
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