Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
Braz. arch. biol. technol
;
60: e17160425, 2017. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-951474
ABSTRACT
ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Tipo de estudio:
Estudio diagnóstico
/
Factores de riesgo
Idioma:
Inglés
Revista:
Braz. arch. biol. technol
Asunto de la revista:
Biologia
Año:
2017
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Italia
Institución/País de afiliación:
Consiglio Nazionale delle Ricerche/IT
/
Universidade Estadual Paulista/BR
/
Universidade Estadual de São Paulo/BR
/
Universidade Federal do Piauí/BR
/
Universita degli Studi di Torino/IT
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