Zeaxanthin from Porphyridium purpureum induces apoptosis in human melanoma cells expressing the oncogenic BRAF V600E mutation and sensitizes them to the BRAF inhibitor vemurafenib
Rev. bras. farmacogn
;
28(4): 457-467, July-Aug. 2018. graf
Artículo
en Inglés
| LILACS
| ID: biblio-958892
ABSTRACT
Abstract Zeaxanthin, an abundant carotenoid present in fruits, vegetables and algae was reported to exert antiproliferative activity and induce apoptosis in human uveal melanoma cells. It also inhibited uveal melanoma tumor growth and cell migration in nude mice xenograft models. Here we report that zeaxanthin purified from the rhodophyte Porphyridium purpureum (Bory) K.M.Drew & R.Ross, Porphyridiaceae, promotes apoptosis in the A2058 human melanoma cell line expressing the oncogenic BRAF V600E mutation. Zeaxanthin 40 µM (IC50) induced chromatin condensation, nuclear blebbing, hypodiploidy, accumulation of cells in sub-G1 phase, DNA internucleosomal fragmentation and activation of caspase-3. Western blot analysis revealed that zeaxanthin induced up-regulation of the pro-apoptotic factors Bim and Bid and inhibition of NF-κB transactivation. Additionally, zeaxanthin sensitized A2058 melanoma cells in vitro to the cytotoxic activity of vemurafenib, a BRAF inhibitor widely used for the clinical management of melanoma, suggesting its potential interest as dietary adjuvant increasing melanoma cells sensitivity to chemotherapy.
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Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Rev. bras. farmacogn
Asunto de la revista:
Farmacia
Año:
2018
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Francia
Institución/País de afiliación:
Federal University of San Francisco Valley/BR
/
Institut Français de Recherche pour l'Exploitation de la Mer/FR
/
University of La Rochelle/FR
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